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Recombinant trypsin

Reagent grade; NLT 3800 USP unit/mg pro
  • CAS Number 9002-07-7
  • Molecular Formula C6H15O12P3
  • Molecular Weight 372.1
  • EC Number 232-650-8
  • Function: replace trypsin ,culture cell
  • Application: produce virus vaccine,Stem cell culture; produce recombinant proteins.
  • Negotiable/10mg,100mg,1g
    Tel:
    +86 21 58183719

    Summary

      Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7.0-9.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF ,aprotinin,soybean
      trypsin inhibitor and by metal chelating agents, e.g.EDTA.It is typically isolated from the pancreas of animals.Animal-derived materials
      are now subject to more strigent regulations, as authorities strive to ensure safer biologics and pharmaceuticals,such as in insulin and
      vaccine production.
      Recombinant porcine trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. Recombinant trypsin is expressed as active protease , with equivalent properties compared to native trypsin .It is manufactured under NSF ISO 9001-2008 and in accordance to good manufacturing practice (GMP) guidelines.No animal derived products are used in the fermentation,purification,or final formulation.

    Properties

      CAS: 9002-07-7
      EC: 3.4.21.4
      Source: Porcine trypsin,expressed in E.coli.

      ADVANTAGES
      Animal origin free:No animal-derived material is used during production.
      Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
      High purity:
      1) Recombinant porcine trypsin provides increased specific activity no less than 3800 USP units/mg pro.
      2) Reduce production costs while improving manufacturing progresses.
      MAIN FEATURES 

      Source E. Coli
      Purified by HPLC
      Format White lyophilized powder
      Specific activity ≥3800 USP units/mg pro
      Purity(RP-HPLC) NLT 70% β-trysin, NMT 20% α-trypsin

      UNIT DEFINITION: One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
      RECOMMEND USAGE 
      Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-9.0. 
      STORAGE INSTRUCTIONS
      Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

    Product Detail

      Function:

      It is used to produce virus vaccine for treating mammalian cells infection.

      Culturing of therapy and primary cells;

      Stem cell culture;

      The production of recombinant proteins.