Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7.0-9.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF ,aprotinin,soybean
trypsin inhibitor and by metal chelating agents, e.g.EDTA.It is typically isolated from the pancreas of animals.Animal-derived materials
are now subject to more strigent regulations, as authorities strive to ensure safer biologics and pharmaceuticals,such as in insulin and
Recombinant porcine trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. Recombinant trypsin is expressed as active protease , with equivalent properties compared to native trypsin .It is manufactured under NSF ISO 9001-2008 and in accordance to good manufacturing practice (GMP) guidelines.No animal derived products are used in the fermentation,purification,or final formulation.
Source: Porcine trypsin,expressed in E.coli.
Animal origin free:No animal-derived material is used during production.
Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
1) Recombinant porcine trypsin provides increased specific activity no less than 3800 USP units/mg pro.
2) Reduce production costs while improving manufacturing progresses.
|Format||White lyophilized powder|
|Specific activity||≥3800 USP units/mg pro|
|Purity（RP-HPLC)||NLT 70% β-trysin, NMT 20% α-trypsin|
UNIT DEFINITION: One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-9.0.
Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.
It is used to produce virus vaccine for treating mammalian cells infection.
Culturing of therapy and primary cells;
Stem cell culture;
The production of recombinant proteins.