Summary
Alkaline phosphatase (ALP), biochemical grade, [9001-78-9], coupled to proteins (antibodies, streptavidin, etc.), can specifically recognize target molecules, and can be used in ELISA, WB and Histochemical testing in progress.
Source: Recombinant Fungi
Classification: EC 3.1.3.1
Molecular weight: 41 kDa (SDS-PAGE)
Buffer system: 30 mM TEA, pH 7.6, 5 mM MgCl2, 0.1 mM ZnCl2, 3 M NaCl
Activator: Mg2+, Zn2+
Optimum pH: 9.8
Stability: Store at 2-8°C for more than 24 months
Technical Indicators
Appearance: Transparent liquid
Specific activity: ≥5000 U/mg
Concentration: ≥10 mg/ml
Purpose
Alkaline phosphatase, when coupled to proteins (such as antibodies, streptavidin, etc.), can specifically recognize target molecules and is suitable for use in ELISA, Western Blot (WB), and histochemical detection.
Alkaline phosphatase can be employed to dephosphorylate the 5'-end of DNA or RNA, preventing self-ligation.
The dephosphorylated DNA or RNA can subsequently be labeled with radioactive phosphate using T4 polynucleotide kinase.