Summary
TRIS-HCl info
Product Code：ACC0029A
Grade: High Purity Grade
CAS No：1185-53-1
Mol. Formula：C4H11NO3HCl
Mol. Weight：157.59
Packing:25KG/DRUM
Purity:99.1%
Appearance：White crystalline powder
Storage:Store at RT. Protect from moisture.
TRIS-HCl introduction
Tris(hydroxymethyl)aminomethane, commonly known as Trometamol, Tris base, or Trizma base, plays a crucial role in diverse research applications as a biological buffer. It is extensively used in the formulation of buffers like Tris-acetate-EDTA (TAE) and Tris-borate-EDTA (TBE), owing to its optimal pKa of 8.1, making it well-suited for maintaining pH within the physiological range (pH 7 -9) applicable to most living organisms. However, caution might be necessary when studying some proteins, as Tris has the potential to interfere with the activity of certain enzymes. Tris base can be employed both independently as a buffer and as a component in mixed buffer formulations, including Tris-EDTA (TE) buffer, TAE buffer, and TBE buffer, among others. Importantly, Tris base is characterized by its purity, essential stability, and relative non-hygroscopic nature.
TRIS-HCl Application
1.Used as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells.
2. Use as a buffer in flow cell sample preparation for Raman spectroscopy.
3. Used as a wash buffer in Western blotting.
4. Used as a buffer component in decellularization of tissues.
5.Used as a buffer in aquaculture field.
Properties
Product Detail
