Guanidine thiocyanate

Guanidine Thiocyanate (Guanidinium thiocyanate or guanidinium isothiocyanate, GITC), a powerful protein denaturant, is most often used to inactivate endogenous RNases in the isolation of RNA from various tissues and bacteria. When used with acid-equilibrated phenol or phenol: chloroform, a solution of Guanidine Thiocyanate and beta-mercaptoethanol are very effective in disrupting both cytoplasmic and nuclear membranes while maintaining the integrity of the RNA.

Purity	≥99.5%
Solubility	Clear and colorless
UV Abs.@280nm	≤0.6 (3M in water)
UV Abs.@300nm	≤0.2 (3M in water)
Melting point	118~121℃
pH(11.8%,water solution)	5.0~7.0
Moisture	≤0.5%
Ash	≤0.5%
Cd	≤5ppm
Co	≤5ppm
Cr	≤5ppm
Cu	≤5ppm
Fe	≤5ppm
Mg	≤5ppm
Mn	≤5ppm
Ni	≤5ppm
Pb	≤5ppm
DNase	Not detected
RNase	Not detected
Appearance	White crystals

Application

Guanidine thiocyanate has been used:

• for the lysis of colorectal adenocarcinoma cell line prior to RNA isolation

• as a PCR inhibitor in triplex mitochondrial DNA (mtDNA) qPCR assay

• for DNA extraction from cerebrospinal fluid (CSF)

Product Category

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