Animal Enteritis modeling reagent-dextran sulfate sodium (DSS)

Ulcerative colitis (UC) is a chronic intestinal inflammatory disease whose etiology and pathogenesis are not fully investigated. The establishment of appropriate colitis animal models is of great significance for studying the etiology, pathogenesis and new treatment methods of UC.

People have made a variety of animal models to study it. Japanese scholars first used dextran sulfate to prepare a mouse UC model in 1985. This method is widely used to screen new treatments for inflammatory bowel disease (IBD) and to develop new preclinical drugs, as well as to study the pathogenesis of IBD. It has the following advantages:

The pathological changes of the DSS-induced UC model are similar to those of human UC;
This model can be applied to both the acute and chronic phases of UC research, allowing experiments to be conducted completely;
Simple, economical and easy to produce.

Seebio Biotech provides dextran sulfate sodium DSS MW: 36000-50000 for colitis modeling, and other molecular weight Dextran Sulfate, Na, molecular weight 5000, 40000, etc.. For more product details, please contact us: service@seebio.cn or Phone: +86 21 58183719 or Wechat: +86 158 0195 7578

Basic information of DSS

Physical properties: white or off-white powder

Description: Dextran sulfate is a polyanionic derivative of dextran, formed by the esterification reaction of dextran and chlorosulfonic acid. The sulfur content is about 17%, which is equivalent to an average of 1.9 sulfate groups in each glucose residual sugar of the dextran molecule.

Solubility: soluble in water (100mg/ml - clear or slightly hazy yellow solution, high molecular weight products are not as soluble in water as low molecular weight products). Before sterilization, the aqueous solution should be formulated into a buffer system (such as sodium bicarbonate) to prevent degradation.

structure:

Important uses of DSS

Improve nucleic acid hybridization rate

When the solution contains 10% dextran sulfate, the re-annealing rate of the DNA chain increases approximately 10 times. This phenomenon further expands the hybridization rate between single-stranded or double-stranded probes and DNA/RNA fixed on the membrane. Not only that, adding 10% dextran sulfate may increase the hybridization rate of randomly cleaved double-stranded DNA probes and immobilized nucleic acids by up to 100 times.

Show immune-related activity

Enhancement and suppression of humoral immunity;
Polyclonal activation of B lymphocytes can even stimulate immature B cells;
In the thymocyte reaction, it promotes conversion into lectin;
Inhibit blood coagulation and platelet aggregation
Enhance fibrinolytic activity;
Enhance/suppress cell-mediated immune response;

Precipitates low-density (LDL) and very low-density (VDLD) lipoproteins

In the presence of Mg 2+ , dextran sulfate precipitates low-density lipoproteins in human serum, leaving high-density lipoproteins in the supernatant. The ability to remove lipoproteins through precipitation of dextran sulfate can also be used to purify other materials, such as beta-2-glycoprotein.

Acts as an adjuvant

Product list of DSS

Item number	product name	average molecular weight
DCJ0018A	Dextran sulfate sodium (DSS)	Mw 5,000
DCJ1606A	Dextran sulfate sodium (DSS)	Mw 40,000
DCJ0105A	Dextran sulfate sodium (DSS)	Mw 36,000~50,000

Use method for reference

Method one:

Mice were given distilled water containing 50g/L dextran sulfate (dextran sulfate sodium) 5000 or 40000 to drink freely for 7 days, and symptoms of acute enteritis such as weight loss, diarrhea, and bloody stools appeared. HE staining of pathological sections revealed diseased colons of mice. The glandular structure was disordered, and the mucosa and submucosal infiltration of mononuclear cells and multinucleated cells was observed. The expression of GSH in the diseased intestinal segment tissue of the DSS group was significantly lower than that of the control group (2±0.6 vs 3.14±1.0, t = 3.95, P = 0.01<0.05). IL-4 secreted by the diseased colon was significantly increased (38.7±4.7 vs 28.7±6.7, t = 3.16, P = 0.009<0.01), and IFN-g was slightly decreased (P>0.05).Source: "The role of GSH in DSS-induced experimental enteritis in mice"

Method Two:

Dissolve DSS with a molecular weight of 50,000 or 40,000 in drinking water to prepare a 3% DSS solution, and let BALB/C mice drink it freely for 7 days to establish a mouse acute colitis model; ② Let BALB/C mice drink 3% DSS solution freely. DSS solution for 7 days, and then drink DSS-free distilled water for 14 days to establish a chronic colitis model in mice; the successful signs of model establishment are semi-loose stools, diarrhea, fecal occult blood (+) and gross bloody stools after drinking 3% DSS for 7 days. Any symptoms.Source: "Establishment and evaluation of mouse colitis model induced by dextran sulfate sodium".

Studies have shown that the characteristics of lesions induced by DSS of different molecular weights are different. For example, mice were fed with DSS of 5000, 40,000 and 500,000. As a result, typical lesions only appeared in the 5,000 and 40,000 groups. The lesions in the 50,000 group were mainly seen in the cecum and upper segment. Colon, in addition, the severity of DSS colitis does not depend on the amount of DSS consumed, but is determined by the DSS concentration. Small differences in the amount of DSS consumed by mice do not affect the severity of induced colitis. Thirdly, different species have different sensitivities to DSS of different molecular weights.

For more product details, please contact us: service@seebio.cn or Phone: +86 21 58183719 or Wechat: +86 158 0195 7578

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