Raw Materials for Cholinesterase Testing

In clinical testing, serum cholinesterase activity measurement is an important tool to aid in the diagnosis of organophosphorus poisoning and assess liver parenchymal cell damage. Its level can also serve as a clinical indicator to determine the severity of the disease and prognosis. Detection methods for cholinesterase include the DGKC (German Society for Clinical Chemistry) method, DTNB method, and dry chemical method. Seebio Biotech provides high-quality raw materials for cholinesterase testing.

 

For more product details, please contact us: service@seebio.cn or Phone: +86 21 58183719 or Wechat: +86 158 0195 7578

 

 

Cholinesterase Classification

Cholinesterase can generally be divided into two categories:

 

true cholinesterase

Distributed in red blood cells and brain gray matter. True cholinesterase, also known as acetylcholinesterase (AChE), mainly acts on acetylcholine and has high specificity

 

pseudocholinesterase

Distributed in liver, brain white matter and serum. Pseudocholinesterase, also known as pseudocholinesterase, includes propionylcholinesterase (PrChE), benzoylcholinesterase (Benzoylcholinesterase, BzChE), butyrylcholinesterase (BuChE) ), usually the main one in serum is butyrylcholinesterase.

 

 

Cholinesterase detection methods and principles

 

DGKC Method

Cholinesterase catalyzes the hydrolysis of butyrylthiocholine to produce butyrate and thiocholine. Thiocholine reduces yellow potassium hexacyanoferrate (Ⅲ) (potassium ferricyanide) into colorless potassium hexacyanoferrate (Ⅱ) (potassium ferrocyanide), and the absorbance decreases at 405nm. Monitored continuously, the rate of decline is directly proportional to the cholinesterase activity in the sample.

 

DTNB Method

The enzyme reaction product, thiocholine, reacts with 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB) to generate 5-mercapto-2-nitrobenzoic acid. This appears yellow at a certain pH, and the color intensity is measured colorimetrically. This method is highly sensitive, requires a small amount of tissue, does not terminate the enzyme reaction, and is suitable for continuous observation of enzyme activity.

 

Dry Chemistry

The enzyme reaction product, thiocholine ester, reacts with DTNB to generate 5-mercapto-2-nitrobenzoic acid. This appears yellow at a certain pH, and the color intensity is measured colorimetrically. This method is highly sensitive, requires a small amount of tissue, does not terminate the enzyme reaction, and is suitable for continuous observation of enzyme activity.

 

 

Cholinesterase Testing Series Raw Materials

 

 

 

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For more product details, please contact us: service@seebio.cn or Phone: +86 21 58183719 or Wechat: +86 158 0195 7578