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Enzyme Raw Materials for Molecular Diagnostics

In Vitro Diagnostics

Molecular diagnostic technology refers to the use of DNA and RNA as diagnostic materials. Through molecular biology techniques, it detects the presence, defects, or abnormal expression of genes. This allows for the diagnosis of the individual's health status and diseases. The basic principle involves examining whether there are structural changes in DNA or RNA, the quantity of these molecules, and whether their expression functions are abnormal. This helps determine whether the individual has any abnormal changes at the genetic level, which is of crucial importance for the prevention, prediction, diagnosis, and prognosis of diseases. In simple terms, all methodological techniques based on the molecular biology level are considered part of molecular diagnostic technology, such as PCR technology, gene sequencing, and more.

Polymerase Chain Reaction (PCR) is a molecular biology technique used to amplify specific nucleotide segments. It can be viewed as a special nucleic acid replication that occurs outside a living organism. PCR is characterized by its ability to significantly amplify minute amounts of nucleic acids, making them easily identifiable and distinguishable.

PCR Technology

PCR can amplify target DNA segments more than a million times. The principle involves using DNA polymerase to catalyze the process, using the parent DNA strand as a template and specific primers as extension starting points. Through denaturation, annealing, extension, and other steps, PCR replicates the process of generating a complementary DNA strand from the parent DNA template outside the living organism.

The standard PCR process consists of three steps:

Denaturation: Utilizing high temperature to separate the DNA double strands. The hydrogen bonds between the DNA double strands are disrupted at elevated temperatures (93-98°C).

Annealing: After the DNA double strands are separated, the temperature is lowered, allowing primers to bind to the single-stranded DNA.

Extension: DNA polymerase starts synthesizing the complementary strand along the DNA chain from the primer-binding site during the temperature drop. Once extension is complete, one cycle is finished, and the number of DNA fragments doubles.

Repeating these three steps 25-35 times exponentially increases the number of DNA fragments.

PCR technology has evolved through continuous technological advancements, including conventional PCR, quantitative fluorescent PCR, digital PCR, and more.

Molecular diagnostic technology has progressed through DNA molecular hybridization, PCR, and biochips, and is now entering the era of gene sequencing. Due to its widespread applications, this industry has experienced rapid global development. In the entire molecular diagnostic industry, as an upstream sector, the product quality and stability of diagnostic materials directly impact the development of downstream products. To better serve the global molecular diagnostic market.

PCR Product List:

Seebio Biotech continually pioneers and innovates in the upstream raw materials field for diagnostic reagents, establishing the Seebio Life Sciences Research Laboratory. The aim is to provide high-quality molecular diagnostic raw materials to the global market, including Seebio® molecular diagnostic materials (enzymes, primers, probes, second-generation sequencing library materials, and modules), along with comprehensive project collaboration solutions. Leveraging expertise in molecular enzyme development and production, Seebio Biotech can offer a range of solutions, from technical validation to research project initiation, laboratory development, product qualification, pilot production, quality control, to product manufacturing, for clients across various sectors such as biotechnology, pharmaceuticals, precision medicine, sequencing, CRO, and more.

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