Guanidine Isothiocyanate - RNA Extraction Reagent

Guanidine isothiocyanate is a denaturing agent frequently employed in RNA isolation procedures. It also serves as a storage buffer for blood samples. This compound effectively inactivates nucleases, making it suitable for the storage and freezing of stool samples for DNA research purposes. In conjunction with phenol-chloroform, guanidine isothiocyanate facilitates RNA extraction.

 

 

Basic Information of Guanidine Isothiocyanate

 

Molecular Formula: CH5N3HSCN

Molecular Weight: 118.16

CAS Registry Number: 593-84-0

 

 

Structural Formula:

 

 

The structural formula of guanidine isothiocyanate, a white crystal that serves as a strong protein denaturant.

 

Guanidine Isothiocyanate in RNA Extraction

 

TRIzol is a comprehensive RNA extraction reagent that can directly isolate total RNA from cells or tissues. Key components of TRIzol include guanidine isothiocyanate and phenol. Guanidinium isothiocyanate effectively lyses cells, facilitates ribosomal dissociation, separates RNA from proteins, and releases RNA into the solution.

 

When chloroform is introduced, it extracts the acidic phenol, leading to the partitioning of RNA into the aqueous phase. Following centrifugation, the aqueous layer (colorless) primarily contains RNA, while the organic layer (yellow) consists primarily of DNA and protein.

 

 

 

 

Applications of Guanidine Isothiocyanate

 

RNA extraction from tissue cells and serum samples, ideal for denaturing nucleic acids and isolating RNA;

As a storage buffer for whole blood samples;

In RNA lysis stabilization buffer formulations, used to lyse colorectal adenocarcinoma cell lines prior to RNA isolation;

Inactivates nucleases, excellent for storing and freezing stool samples for DNA research;

As a PCR inhibitor in mitochondrial DNA (mtDNA) qPCR reaction systems;

For qPCR analysis of DNA extracted from cerebrospinal fluid (CSF).

 

 

Quality Index

 

 

Product List

 

Seebio Biotech supplies guanidine isothiocyanate with excellent quality and stable quality. For more product details, please contact us: service@seebio.cn or Phone: +86 21 58183719 or Wechat: +86 158 0195 7578

 

 

References

[1] Xu Jie, Li Mingqi. Improvement of certain operating techniques in the one-step RNA extraction using guanidinium isothiocyanate-phenol-chloroform. "Plant Physiology Letters"》，1996
[2] Xu Jie, Li Mingqi. Improvement of certain operating techniques in the guanidinium isothiocyanate-phenol-chloroform-step method for RNA extraction. 《CNKI》，1996
[3] Wang Yaohe. One-step extraction of RNA from tissues using guanidinium isothiocyanate-phenol-chloroform. "Journal of Zhengzhou University (Medical Edition)")》，1997
[4] Yang Lili, Liu Hongying, Yang Hejun, et al. An improved method for extracting trace amounts of fetal RNA from peripheral blood of pregnant women using the guanidine isothiocyanate method combined with a silica membrane centrifugal adsorption column. "Chinese Journal of Biochemistry and Molecular Biology"》，2012
[5] Hu Guobin, Mei Xingguo, Liu Yi. Improved one-step method of guanidine isothiocyanate extraction of RNA from yew cells. 《CNKI》，2001
[6] RNA Extraction from Plant Tissue with Homemade Acid Guanidinium Thiocyanate Phenol Chloroform (AGPC), Current protocols, 2022/1
[7] Evaluation of Alternative Transport Media for RT-qPCR-Based SARS-CoV-2 Testing, International journal of analytical chemistry, 2022/1
[8] Evaluation of stability and inactivation methods of SARS-CoV-2 in context of laboratory settings, Medical microbiology and immunology, 2021/8
[9] Applicability of polyethylene glycol precipitation followed by acid guanidinium thiocyanate-phenol-chloroform extraction for the detection of SARS-CoV-2 RNA from municipal wastewater, The Science of the total environment, 2021/2
[10] Inactivation of SARS-CoV-2 virus in saliva using a guanidium based transport medium suitable for RT-PCR diagnostic assays, medRxiv : the preprint server for health sciences, 2021/1
[11] Methods to disinfect and decontaminate SARS-CoV-2: a systematic review of in vitro studies, Therapeutic advances in infectious disease, 2021/1
[12] Inactivation of SARS-CoV-2 virus in saliva using a guanidium based transport medium suitable for RT-PCR diagnostic assays, PloS one, 2021/1