Commonly Used Raw Materials and Excipients for Hematology Reagents
Time:2024-01-31 Hits:266
In the blood analysis process of a hematology analyzer, the use of various supporting reagents is crucial, and each step influences the attainment of accurate results. For instance, substandard physical and chemical indicators in the diluent can lead to elevated platelet (PLT) background, poor white blood cell (WBC) classification, and hematocrit (HCT) drift. Hemolytic agents with incomplete or excessive hemolysis can distort white blood cell count results, while cleaning agents impact the instrument's blank value and blanking rate. Apart from the formulation ratio, the selection of raw materials is a pivotal factor influencing these effects.
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Common Classifications of Hematology Reagents
Hematology analyzers typically employ three key reagents: diluent, hemolytic agent, and cleaning solution.
1 Diluent
Blood cell diluent is commonly an isotonic solution with acid-base buffering effects, utilizing buffers such as ADA, TRIS, HEPES, imidazole, etc. It maintains appropriate ionic strength and conductivity. Diluent is vital for blood cell analyzers in clinical testing, playing a crucial role in blood cell classification or counting. Additionally, it collaborates with the cleaning solution before analysis to reduce instrument background values and prevent interference from previous test samples. Diluent also helps preserve the original shape of white blood cells and platelets, ensuring accurate classification by the instrument.
2 Hemolytic Agent
A hemolytic reagent is essential for counting white blood cells and determining hemoglobin (HGB) content. It dissolves red blood cells, releasing hemoglobin, which combines with components in the hemolytic agent to form derivatives. Colorimetry at a specific wavelength, usually 530 to 550nm, measures the absorbance change, directly proportional to hemoglobin content. Hemolytic agents induce differences among white blood cell types, enabling the instrument to classify and count various groups.
3 Cleaning Fluid
Cleaning fluid is designed to cleanse the instrument pipeline of blood and remove proteins, minimizing cross-contamination and ensuring result reliability and accuracy. It creates a humid environment, swiftly eliminating air bubbles from the entire flow system. Ingredients often include high-quality amphoteric surfactants, animal hydrolytic proteases, preservatives, sodium formate, sodium chloride, and buffers such as ADA, TRIS, HEPES, and imidazole. The cleaning agent is indispensable for the hematology analyzer, a crucial factor in obtaining accurate test results.
4 Other Reagents
Additional reagents used in blood cell analysis include anticoagulants. The International Committee for Standardization (ICSH) recommends EDTA as the preferred anticoagulant for blood samples in counting and screening blood cells.
Accurate test results depend on the correct use of these analysis reagents.
Product List
Item number
|
Product name
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CAS No.
|
Buffer
|
||
ACC0013A
|
4-(2-Hydroxyethyl)-1-piperazineeth anesulfonic acid
|
7365-45-9
|
ACC0030B
|
Tris(hydroxymethyl) aminomethane,tris base
|
77-86-1
|
ACC0036A
|
Tris(hydroxymethyl) aminomethane,tris base
|
64431-96-5
|
AHD0006A
|
1,3-Bis[tris(hydroxymethyl)methylamino]propane
|
1303-96-4
|
AHB0001A
|
Boric acid
|
10043-35-3
|
AHD0031A
|
Sodium phosphate monobasic dihydrate
|
13472-35-0
|
ACP0005A
|
Glycine
|
56-40-6
|
AJL0030D
|
Maleic acid
|
110-16-7
|
Quaternary ammonium salt
|
||
ACH0168A
|
n-Octyl trimethyl ammonium chloride
|
10108-86-8
|
ACH0170A
|
n-Decyl trimethyl ammonium chloride
|
10108-87-9
|
ACH0020A
|
Dodecyl trimethyl ammonium chloride
|
112-00-5
|
ACH0019A
|
Dodecyl trimethyl ammonium bromide
|
1119-94-4
|
ACH0021A
|
Tetradecyl trimethyl ammonium chloride
|
|
ACH0022A
|
Tetradecyl trimethyl ammonium bromide
|
1119-97-7
|
ACH0024A
|
Cetyl trimethyl ammonium chloride
|
112-02-7
|
ACH0023A
|
Cetyl trimethyl ammonium bromide
|
57-09-0
|
Surfactant
|
||
ACH0017B
|
Sodium dodecyl sulfate
|
151-21-3
|
ACH0026A
|
Surfactant S14
|
|
ACH0028A
|
Surfactant S20
|
|
ACH0003B
|
Surfactant S21
|
|
ACH0107A
|
Surfactant S23
|
|
ACH0010A
|
Ethylenediaminetetraacetic acid disodium salt dihydrate
|
6381-92-6
|
ACH0008A
|
Dipotassium salt dihydrate
|
25102-12-9
|
ACC0015A
|
288-32-4
|
|
ACH0113A
|
Emulsifier AEO-9
|
|
ACH0376A
|
Emulsifier OP-10
|
|
Preservative
|
||
ACN0046A
|
Krovin 500
|
|
ACN0047B
|
Krovin 600
|
|
ACN0043B
|
Krovin 950
|
|