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Creatine Kinase Isoenzyme (CK-MB) - Magnetic Particle Chemiluminescence Method (Acridinate)/Fluorescence Immunochromatography Solution

Time:2024-02-20 Hits:195

Project Background

CK-MB (Creatine Kinase MB) is one of the three isoenzymes of creatine kinase (CK), alongside CK-BB and CK-MM. CK serves as a key enzyme in muscle metabolism, facilitating the reversible phosphorylation of creatinine through adenosine triphosphate (ATP). CK-MB, composed of two subunits (MW=40,000 each), features subunit M for muscle and subunit B for brain.

Originally, immunoassay techniques were employed to measure CK-MB activity and compare it to total CK activity, expressed as the CK-MB/CK ratio. However, challenges arose with the presence of CK-BB, adenylyl cyclase (AK), and atypical CK (large CK), which could not be neutralized by anti-M antibodies, potentially leading to excessively high CK-MB results.

Currently, many enzyme immunoassay techniques for quantifying CK-MB levels (ng/mL) are closely aligned with CK-MB activity measurement and are not interfered by CK-BB, atypical CK, and AK.


Performance

Chemiluminescence (Acridinium Ester) Platform:

Combination 1: Creatine Kinase Isoenzyme (CK-MB) Antibody (Cat. No.: EKY0095J) coated carboxyl magnetic beads + Creatine Kinase Isoenzyme (CK-MB) Antibody (Cat. No.: EKY0095K) labeled acridinium ester;
Linear range: 0.3ng/mL-300ng/mL;




Combination 2: Creatine Kinase Isoenzyme (CK-MB) Antibody (Cat. No.: EKY0095K) coated carboxyl magnetic beads + Creatine Kinase Isoenzyme (CK-MB) Antibody (Cat. No.: EKY0095J) labeled acridinium ester;
Linear range: 0.3ng/mL-300ng/mL;

Fluorescence Immunochromatography (Lanthanide Eu Chelate Filling) Platform:

Combination:Creatine Kinase Isoenzyme (CK-MB) Antibody (Cat. No.: EKY0095J) labeled fluorescent microspheres + Creatine Kinase Isoenzyme (CK-MB) Antibody (Cat. No.: EKY0095K) for streaking;
Linear range: 0.5ng/mL-100ng/mL.


Clinical Relevance

Chemiluminescence (Acridinium Ester) Platform:
The clinical correlation between the high-value part and R brand CK-MB reagent R2>0.98;
The correlation between the low-value part (15ng/mL) and R brand CK-MB reagent R2>0.96.






Fluorescence Immunochromatography (Lanthanide Eu Chelate Filling) Platform:
The clinical correlation between the high-value part and R brand CK-MB reagent R2>0.97;
The correlation between the low-value part (20ng/mL) and R brand CK-MB reagent R2>0.95.



Test Program

Chemiluminescence (Acridinium Ester) Platform:

Combination 1:
Antigen to be tested:CK-MB antigen (Cat. No.: EDD0408B), sample/standard adding volume: 20ul;
Reagent 1: Creatine Kinase Isoenzyme (CK-MB) Antibody (Cat. No.: EKY0095J) coated-magnetic bead dilution, dosage 50ul/test;
Reagent 2: Creatine Kinase Isoenzyme (CK-MB) Antibody (Cat. No.: EKY0095K) labeled-acridinate dilution, dosage 50ul/test;

Combination 2:
Antigen to be tested: CK-MB antigen (Cat. No.: EDD0408B), sample/standard adding volume: 20ul;
Reagent 1: Creatine Kinase Isoenzyme (CK-MB) Antibody (Cat. No.: EKY0095K) coated-magnetic bead dilution, dosage 50ul/test;
Reagent 2: Creatine Kinase Isoenzyme (CK-MB) Antibody (Cat. No.: EKY0095J) labeled-acridinate dilution, dosage 50ul/test;
Luminescence instrument used in the test: Cosmai SMART 500S; a chemiluminescence instrument compatible with various acridinium ester systems (such as Sichuan Mike, Shenzhen Yahuilong, Shandong Kanghua Bio, Getein Bio, Nanjing Norman, etc.).


Fluorescence Immunochromatography (Lanthanide Eu Chelate Filling) Platform:
Antigen to be tested:CK-MB antigen (Cat. No.: EDD0408B), sample/standard adding volume: 10ul;
Reaction conditions: mix sample + fluorescent solution, load sample, reaction time (10min-15min);
Fluorescence instrument used for testing: Hemai FIC-S100; an instrument compatible with various fluorescence systems (excitation wavelength 365nm, emission wavelength 615nm) (such as Guangzhou Labsim, etc.).


Product Information
Product
Item number
Recommended pairing
Creatine kinase isoenzyme (CK-MB) antibody
EKY0095J
Creatine kinase isoenzyme (CK-MB) antibody (Cat. No.: EKY0095J) coated carboxyl magnetic beads + Creatine kinase isoenzyme (CK-MB) antibody (Cat. No.: EKY0095K) labeled acridinium ester(Luminous Pairing Set1)
Creatine kinase isoenzyme (CK-MB) antibody (Cat. No.: EKY0095K) coated carboxyl magnetic beads + Creatine kinase isoenzyme (CK-MB) antibody (Cat. No.: EKY0095J) labeled acridinium ester(Luminous Pairing Set2)
Creatine kinase isoenzyme (CK-MB) antibody (Cat. No.: EKY0095J) labeled fluorescent microspheres + Creatine kinase isoenzyme (CK-MB) antibody (Cat. No.: EKY0095K) streaking(Fluorescence pair combination)
Creatine kinase isoenzyme (CK-MB) antibody
EKY0095K
Creatine kinase isozyme (CK-MB) antigen
EDD0408B
Creatine kinase isoenzyme (CK-MB) antibody (Cat. No.: EKY0095J)-Carboxyl Magnetic Bead Concentrate
DKY1312A
Creatine kinase isoenzyme (CK-MB) antibody (Cat. No.: EKY0095K) - Acridinium Ester Concentrate
DKY1312B
Creatine kinase isoenzyme (CK-MB) antibody (Cat. No.: EKY0095K)-Carboxyl Magnetic Bead Concentrate
DKY1312C
Creatine kinase isoenzyme (CK-MB) antibody (Cat. No.: EKY0095J) - Acridinium Ester Concentrate
DKY1312D
Creatine kinase isoenzyme (CK-MB) antibody (Cat. No.: EKY0095J) labeled fluorescent microsphere concentrate
DKY1312E
Carboxy magnetic beads (superparamagnetic), 1um
DEG0091B-1-100mg
Carboxy magnetic beads (superparamagnetic), 3um
DEG0091A-3-100mg
Time-resolved fluorescent microspheres, carboxyl (200nm)
AEG0786B

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