Universal nuclease

Multifunctional nuclease, cGMP grade, [9025-65-4], is a genetically engineered endonuclease from Serratia marcescens and is a typical representative of multifunctional nucleases commonly used in the production process of biological products.

It exhibits the capability to degrade various forms of DNA and RNA, including single-stranded, double-stranded, linear, superhelical, and circular DNA, RNA, and genomic DNA. The resultant breakdown yields smaller oligonucleotides, primarily composed of 2-5 bases, without displaying specificity for nucleic acid sequences and lacking protease activity.

Key Characteristics

Molecular Weight: 27 kDa (monomer with two identical subunits)
Suitable pH: pH 8.0~9.0
Suitable Temperature: 37℃
Isoelectric Point: pH 6.2
Cofactor: Mg2+
Product Presentation:

The totipotent nuclease is supplied in liquid form, and its formulation comprises:

20mM Tris hydrochloride buffer at pH 8.2
20mM sodium chloride
2mM magnesium chloride
50% glycerol (v/v)