Polysucrose 400 (Ficoll 400), white crystalline powder. soluble in water. Stable in neutral and alkaline solutions. Hydrolysis breaks down at pH<3. Using sucrose and epichlorohydrin as raw materials, it is a synthetic water-soluble polymer made by the copolymerization of sucrose and 3-chloro-1,2-propylene oxide, with a molecular weight of about 4×105. Widely used as a medium for density gradient centrifugation. It can also be used as an inert carrier for low molecular weight haptens in immunological research. Ficoll-400 is often used, that is, the relative molecular weight is 400,000. Polysucrose 400 has a low osmotic pressure, but its viscosity is very high. For this reason, it is often mixed with diatrizoate to reduce the viscosity.
Polysucrose 400 is mainly used for the density gradient separation of cells, cell membranes and virus cells, and can also be used to separate different organelles.
use
1) Application in food field
Polysucrose 400 is mainly used for thickening, emulsifying, stabilizing and other functions. It can be used as an additive in desserts, beverages, dairy products, meat products, salad dressings and other foods to improve their taste and texture. At the same time, polysucrose 400 is also a low-calorie, low-calorie additive, so it is widely used in some low-calorie foods.
2) Application in the pharmaceutical field
Polysucrose 400 is mainly used in the preparation of oral tablets, capsules, liquid preparations and other drugs. It can be used as a stabilizer, thickener, release control agent, etc., which can improve the taste and texture of the drug, and help control the release rate of the drug and improve the efficacy of the drug.
3) Application in the field of cosmetics
Polysucrose 400 is mainly used in the preparation of cosmetics such as lotion, face cream, and shampoo. It can be used as thickener, emulsifier, moisturizer, etc., which can improve the consistency, stability and moisturizing performance of cosmetics. At the same time, polysucrose 400 is also a mild ingredient, non-irritating to the skin, so it is widely used in some sensitive skin and baby skin care products.
