Special Quality Control Raw Materials for Colloidal Gold C-Line: Chicken IgY and Rabbit Anti-Chicken IgY Antibodies

Immunoglobulin Y (IgY) constitutes the predominant immunoglobulin in birds, amphibians, and reptiles.

 

Background on Immunoglobulin Yolk (IgY): Yolk antibodies (IgY) are acquired from the yolks of laying hens through immunization. This innovative approach, known as yolk antibody preparation, has experienced rapid advancements in recent years. IgY exhibits stable properties, high yield, and cost-effectiveness, aligning with animal welfare requirements. Leveraging its advantage of a significant phylogenetic distance from mammals, IgY is well-suited for specific antibody production. It has found widespread applications in biological and medical domains, particularly in the development of diagnostic POCT kits. Seebio Biotechnology, renowned for the well-received Quality Control Line C, has been dedicated to the IVD field for almost two decades. The company has developed chicken IgY and rabbit anti-chicken/goat anti-chicken IgY products that align with colloidal gold projects, enhancing and complementing the Seebio brand in chromatography. For inquiries and orders related to the C-Line product family on the platform (including rabbit IgG, goat anti-rabbit IgG, and DNP antigen antibodies), please contact us: service@seebio.cn or Phone: +86 21 58183719 or Wechat: +86 158 0195 7578.

 

 

Differences and Advantages Between IgY and IgG

 

 

IgY shares similarities with mammalian IgG. The molecular weight of normal chicken IgY is approximately 180KDa, consisting of two light chains (2L) and two heavy chains (2H), with molecular weights of 60-70KDa and 22-30KDa, respectively. Its isoelectric point is around 5.2. In comparison to general mammalian IgG, IgY demonstrates superior heat resistance, acid resistance, ionic strength resistance, and some resistance to enzyme degradation.

 

 

Application of IgY Products in In Vitro Diagnostic Projects

IgY serves as an ideal tool for immunological experimental detection. It enables the preparation of specific antibodies against particular pathogenic organisms for pathogen isolation and identification. Notably, IgY does not bind to rheumatoid factor, and it avoids interaction with protein A, protein G, and mammalian FC. It binds to the body and complement without significant cross-reactivity with IgG. Consequently, IgY is recommended for the specific determination of circulating immune complexes, rheumatoid factors, complement, etc., minimizing the occurrence of false positives.

 

The quality of the C-Line in POCT detection reagents is of utmost importance. It facilitates timely, accurate, and intuitive assessments of the effectiveness of detection reagent products, batch-to-batch consistency and stability, and the precision of sample quantification. Common quality control systems (labeled-coated) in POCT detection reagents include rabbit IgG-goat anti-rabbit IgG, chicken IgY-rabbit/goat anti-chicken IgY, and independent quality control DNP-goat anti-DNP, among others.

 

 

 

Chicken IgY 

Item Number: DCL0114B

Specifications: 5mg

Appearance: Solution

Purity: >90% (SDS-PAGE)

Molecular Weight: 180KD

Buffer: 0.01M PBS pH7.4, 0.1% ProClin 300

Application: Specifically designed for Quality Control C-Line

 

 

Rabbit Anti-Chicken IgY

Item Number: DCY0630B

Specifications: 5mg

Appearance: Solution

Purity: >90% (SDS-PAGE)

Buffer: 0.01M PBS pH7.4, 0.1% Proclin 300

 

 

Application: Quality Control C-Line

 Advantages of Chicken IgY for C-Line Quality Control:

 

1 Higher Affinity: Chickens, being non-mammals, exhibit an enhanced response to mammalian antigens, resulting in antibodies with higher affinity.

 2 Ease of Obtaining: Chicken IgY can be easily obtained in large quantities from eggs without the need for blood collection.

 3 Fast Preparation with Short Cycle: Compared to rabbits, high-titer antibodies can be obtained from eggs as early as the 25th day of immunization.

 4 Easy Mass Production and Control: Chicken IgY mass production is easily controllable, and stable, non-specific antigen-binding antibodies can be reliably obtained from Specific Pathogen-Free (SPF) eggs.

 5 Chicken IgY Cross-Reactivity: The likelihood of serological cross-reaction with mammalian immunoglobulins is low due to the substantial germline distance between the two.

 6 Lower Background with IgY Antibodies: IgY-Fc contains two sugar side chains, in contrast to IgG-Fc, which contains one sugar side chain. The distinct amino acid compositions contribute to functional differences. IgY does not bind to human rheumatoid factor, protein A, or protein G. Additionally, it does not activate the mammalian complement system, thereby preventing false-negative or false-positive results during immune detection.

 

7 Acid Resistance and Stability: IgY is acid-resistant, remaining active at pasteurization temperatures and maintaining stability at pH levels below 3.

 

8 Convenient Storage and Transportation: IgY antibodies offer more stable, convenient, and reliable storage and transportation compared to antibodies extracted from mammals.

 

Seebio Biotech's Production Technology:

 

Seebio Biotech employs mature and stable production technology, ensuring a consistent supply of high-quality chicken IgY.

Related Products:

 

High-Quality Independent C-Line Quality Control System - DNP Antigen and Antibody

 

 

 

Seebio Biotech Solutions:

Seebio Biotech provides comprehensive solutions for secondary antibody products dedicated to quality control C-Line immunoglobulins. For more product details, please contact us: service@seebio.cn or Phone: +86 21 58183719 or Wechat: +86 158 0195 7578