Heparin Binding Protein (HBP) Magnetic Particle Chemiluminescence Method (Acridinium Ester)/Fluorescence Immunochromatography Solution
Time：2024-01-24 Hits：257
Background of the Project
Heparin-binding protein (HBP) is a granule protein derived from mature neutrophils, primarily stored in azurophilic granules with a small fraction in secretory vesicles. HBP, a member of the serine protease family, lacks protease activity but exhibits high affinity for lipopolysaccharide lipid A.
Upon neutrophil activation, HBP is released during degranulation. In cases of acute bacterial infection, blood HBP concentration can significantly increase within 1-2 hours, while in viral infections, HBP either does not increase or increases only slightly. HBP levels also act as a prognostic indicator, decreasing rapidly with effective treatment. Thus, detecting HBP in patients' blood aids in clinical diagnosis, prediction of acute bacterial infections, assessment of infection severity, and monitoring antibiotic efficacy. Compared to traditional inflammation markers, HBP rises more rapidly and demonstrates better specificity during bacterial infections.
Clinical Applications of HBP:
a) Auxiliary diagnosis and differential diagnosis of infectious diseases;
b) Positive correlation of HBP concentration with infection severity, useful for assessing the severity of infection;
c) Auxiliary diagnosis of sepsis. HBP is recommended by the "Chinese Severe Sepsis/Septic Shock Treatment Guidelines (2014)" and the "China Expert Consensus on Early Prevention and Intervention of Sepsis 2020" as an excellent indicator for diagnosing sepsis.
d) Sepsis prognosis assessment. HBP, with a half-life of less than 1 hour, quickly reflects the patient's therapeutic effect and is closely related to sepsis prognosis as a pathogenic factor.
b) Positive correlation of HBP concentration with infection severity, useful for assessing the severity of infection;
c) Auxiliary diagnosis of sepsis. HBP is recommended by the "Chinese Severe Sepsis/Septic Shock Treatment Guidelines (2014)" and the "China Expert Consensus on Early Prevention and Intervention of Sepsis 2020" as an excellent indicator for diagnosing sepsis.
d) Sepsis prognosis assessment. HBP, with a half-life of less than 1 hour, quickly reflects the patient's therapeutic effect and is closely related to sepsis prognosis as a pathogenic factor.
Performance
Chemiluminescence (Acridinium Ester) Platform:
Combination: Heparin-binding protein (HBP) monoclonal antibody (Cat. No.: EKY0855A) coated carboxyl magnetic beads + Heparin-binding protein (HBP) monoclonal antibody (Cat. No.: EKY0856A) labeled acridinium ester;
Linear range: 5.9 ng/mL-300 ng/mL;
Linear range: 5.9 ng/mL-300 ng/mL;
Fluorescence Immunochromatography (Lanthanide Eu Chelate Filling) Platform:
Combination: Heparin-binding protein (HBP) monoclonal antibody (Cat. No.: EKY0856A) labeled fluorescent microspheres + Heparin-binding protein (HBP) monoclonal antibody (Cat. No.: EKY0855A) for streaking;
Linear range: 5.9 ng/mL-300 ng/mL.
Linear range: 5.9 ng/mL-300 ng/mL.
Clinical Relevance
Chemiluminescence (Acridinium Ester) Platform:
Clinical correlation with ZH brand HBP reagent R² > 0.95;
Fluorescence Immunochromatography (Lanthanide Eu Chelate Filling) Platform:
Clinical correlation with ZH brand HBP reagent R² > 0.94.
Test Program
Chemiluminescence (Acridinium Ester) Platform:
Antigen to be tested: Heparin-binding protein antigen (HBP antigen) (Cat. No.: EDD0159B/EDD0159C), sample/standard adding volume: 10 ul;
Reagent 1: Heparin-binding protein (HBP) monoclonal antibody (Cat. No.: EKY0855A) coated-magnetic bead dilution, dosage 50 ul/test;
Reagent 2: Heparin-binding protein (HBP) monoclonal antibody (Cat. No.: EKY0856A) labeled-acridinate dilution, dosage 50 ul/test;
Luminescence instrument used in the test: Cosmai SMART 500S; chemiluminescence instrument suitable for various acridinium ester systems (such as Sichuan Mike, Shenzhen Yahuilong, Shandong Kanghua Bio, Getein Bio, Nanjing Norman, etc.).
Reagent 1: Heparin-binding protein (HBP) monoclonal antibody (Cat. No.: EKY0855A) coated-magnetic bead dilution, dosage 50 ul/test;
Reagent 2: Heparin-binding protein (HBP) monoclonal antibody (Cat. No.: EKY0856A) labeled-acridinate dilution, dosage 50 ul/test;
Luminescence instrument used in the test: Cosmai SMART 500S; chemiluminescence instrument suitable for various acridinium ester systems (such as Sichuan Mike, Shenzhen Yahuilong, Shandong Kanghua Bio, Getein Bio, Nanjing Norman, etc.).
Fluorescence Immunochromatography (Lanthanide Eu Chelate Filling) Platform:
Antigen to be tested: Heparin-binding protein antigen (HBP antigen) (Cat. No.: EDD0159B/EDD0159C), sample/standard adding volume: 5 ul;
Reaction conditions: Mix sample + fluorescent solution, load sample, reaction time (10 min-15 min);
Fluorescence instrument used in the test: Hemai FIC-S100; an instrument applicable to various fluorescence systems (excitation wavelength 365nm, emission wavelength 615nm) (such as Guangzhou Labsim, etc.).
Reaction conditions: Mix sample + fluorescent solution, load sample, reaction time (10 min-15 min);
Fluorescence instrument used in the test: Hemai FIC-S100; an instrument applicable to various fluorescence systems (excitation wavelength 365nm, emission wavelength 615nm) (such as Guangzhou Labsim, etc.).
Product Information
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Product
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Item number
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Recommended pairing
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Heparin-binding protein (HBP) monoclonal antibody
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EKY0855A
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Heparin-binding protein (HBP) monoclonal antibody (Cat. No.: EKY0855A) coated carboxyl magnetic beads + Heparin-binding protein (HBP) monoclonal antibody (Cat. No.: EKY0856A) labeled acridinium ester（Luminous Pairing Set）
Heparin-binding protein (HBP) monoclonal antibody (Cat. No.: EKY0856A) labeled fluorescent microspheres + Heparin-binding protein (HBP) monoclonal antibody (Cat. No.: EKY0855A) streaking（Fluorescence pair combination） |
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Heparin-binding protein (HBP) monoclonal antibody
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EKY0856A
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Heparin binding protein antigen (HBP antigen）
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EDD0159B
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Heparin binding protein antigen (HBP antigen）
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EDD0159C
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Heparin-binding protein (HBP) monoclonal antibody (Cat. No.: EKY0855A)-carboxy magnetic bead concentrate
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DKY1313A
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Heparin-binding protein (HBP) monoclonal antibody (Cat. No.: EKY0856A)-Acridinium ester concentrate
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DKY1313B
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Heparin-binding protein (HBP) monoclonal antibody (Cat. No.: EKY0856A) labeled fluorescent microsphere concentrate
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DKY1313C
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Carboxy magnetic beads (superparamagnetic), 1um
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DEG0091B-1-100mg
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Carboxy magnetic beads (superparamagnetic), 3um
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DEG0091A-3-100mg
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Time-resolved fluorescent microspheres, carboxyl (200nm）
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AEG0786B
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