Lacto-N-Triose: Safeguarding Neonatal Gut Health

Lacto-N-Triose (LNT II) is a core structure of human milk oligosaccharides (HMOs) with extensive potential for nutritional and health benefits. LNT II, along with HMOs, plays a vital role in newborns' gut health. It not only acts as a key player in infection resistance, aiding the newborn's intestine in defending against invading pathogenic microorganisms to maintain overall health, but also helps maintain gut microbiota balance. It promotes the growth of beneficial probiotics while limiting the proliferation of harmful microorganisms, ensuring a stable gut environment. These attributes make Lacto-N-Triose an essential asset in infant nutrition and health, providing comprehensive support for neonatal gut health.

Seebio Biotech offers food-grade Lacto-N-Triose. Please contact: service@seebio.cn or Phone: +86 21 58183719.

Basic Product Information

Lacto-N-Triose

Molecular Formula	C20H35O16N
Molecular Weight	545.48
State	Powder
pH (20°C, 5% solution)	5.0-7.0

Application Areas:

Suitable for functional foods, infant formula, specialty medical foods, nutritional supplements, beverages, pharmaceuticals, and research reagents.

Infant Formula and Functional Food Additive: Lacto-N-Triose II (LNT II), an essential human milk oligosaccharide, is the precursor of lacto-N-tetraose and lacto-N-neotetraose, and its safety has been evaluated. In a 90-day experiment, LNT II was administered at different dose levels (0, 1,500, 2,500, or 5,000 mg/kg body weight) and followed by a 4-week untreated recovery period. In vitro experiments showed no genotoxicity for LNT II. The highest dose of 5,000 mg/kg body weight/day was determined as the No Observed Adverse Effect Level (NOAEL). These study results suggest that LNT II can be safely used in infant formula and food components within the range naturally present in human breast milk.

Promotion of Beneficial Bacteria: The consumption of Lacto-N-Triose has a positive impact on the balance of the human gut microbiota. Lacto-N-Triose enhances the adhesion of intestinal epithelial cells, promoting the production of succinic acid and short-chain fatty acids. This not only contributes to the survival and growth of beneficial bacteria but also reduces the population of pathogenic bacteria.

Protecting Newborns: In human milk oligosaccharides, Lacto-N-Triose demonstrates a significant inhibitory effect on the growth of Group B Streptococcus (GBS) bacteria. This implies that the intake of Lacto-N-Triose can assist newborns in resisting various potential risks.

Regulating the Human Immune System: LNT2 can induce the production of IL-10 and TNF-α, with its action depending on NF-κB. Compared to other human milk oligosaccharides (HMOs), the effect of LNT2 is more pronounced. This may be attributed to LNT2's unique N-acetylglucosamine end structure and its binding to Toll-like receptors (TLR) rich in leucine repeats. This suggests that HMOs' impact on macrophage TLR signaling and immune regulation depends to some extent on their structure.

Reducing Food Allergy Risk: Lacto-N-Triose can directly prevent pathogenic microorganisms or toxins from binding to receptors on the surface of intestinal epithelial cells through its terminal sugar chains. This effectively reduces the risk of food allergies.

For more information about Seebio Biotech products, please contact: service@seebio.cn or Phone: +86 21 58183719.

References:

[1] Lianghui Cheng,et al. Human Milk Oligosaccharides Differently Modulate Goblet Cells Under Homeostatic, Proinflammatory Conditions and ER Stress. DOI:10.1002/mnfr.201900976
[2] Lianghui Cheng,et al. Human milk oligosaccharides and its acid hydrolysate LNT2 show immunomodulatory effects via TLRs in a dose and structure-dependent way. DOI:10.1016/J.JFF.2019.05.023
[3] Chunli Kong,et al. Distinct fermentation of human milk oligosaccharides 3-FL and LNT2 and GOS/inulin by infant gut microbiota and impact on adhesion of Lactobacillus plantarum WCFS1 to gut epithelial cells.. DOI:10.1039/d1fo02563e
[4] Chunli Kong,et al. Human Milk Oligosaccharides Mediate the Crosstalk Between Intestinal Epithelial Caco-2 Cells and Lactobacillus PlantarumWCFS1in an In Vitro Model with Intestinal Peristaltic Shear Force. DOI:10.1093/jn/nxaa162
[5] Lianghui Cheng,et al. Effects of Different Human Milk Oligosaccharides on Growth of Bifidobacteria in Monoculture and Co-culture With Faecalibacterium prausnitzii. DOI:10.3389/fmicb.2020.569700
[6] Kong, C, et al. Human milk oligosaccharides mediate the crosstalk between intestinal epithelial Caco-2 Cells and Lactobacillus Plantarum WCFS1in an in vitro model with intestinal peristaltic shear force. doi: 10.1093/jn/nxaa162
[7] Xu Fang,et al. Preclinical Safety Evaluation of the Human-Identical Milk Oligosaccharide Lacto-N-Triose II. DOI: 10.1177/10915818231203515

 

 

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