Seebio Recombinant Human Type III Collagen with High Activity

Collagen, a crucial protein abundant in the human body, is found throughout various tissues, including skin, bones, muscles, blood vessels, and internal organs. It provides essential structural support. Among the 28 identified collagen types, Type I, Type II, and Type III constitute over 90% of total body collagen, with Type I and Type III being prominent in skin tissue. Collagen's ubiquity and structural significance make it a valuable biomaterial for regenerative medicine and tissue engineering. Ongoing research aims to develop recombinant human collagen for applications like skin fillers, wound healing, and cartilage repair, with a focus on optimizing expression, purification, and bioactivity enhancement. This work holds great promise for improving human health.

Seebio provides highly active recombinant human Type III collagen. For more product details, please contact us: service@seebio.cn or Phone: +86 21 58183719 or Wechat: +86 158 0195 7578

Collagen has a right-handed triple helical structure, where each peptide chain is left-handed. These three left-handed α-chains intertwine to form the right-handed helical structure of collagen, known as the collagen triple helix. These helices aggregate to form a mesh-like structure, providing strong elasticity and robust support, offering dual functions. The structure of a protein determines its function.

Skin's collagen content reaches its peak between the ages of 20 and 30, after which collagen begins to degrade, decreasing at a rate of 1% to 1.5% annually. The content of Type III collagen begins to decline from birth.

Sources of Collagen:

Plant-Based Collagen: All collagen originates from animals, and there is no such tissue in plants, nor proteins of that kind. So-called "plant collagen" refers to sugar proteins derived from plants such as peach gum and Tremella fuciformis, but they are not true collagen. Recombinant technologies may utilize plants like tobacco as host cells to produce animal collagen but are not genuinely plant-derived.

Animal-Based Collagen: 99% of commercially available collagen is extracted from animal tissues such as pigskin, cowhide, donkey skin, fish skin, and fish scales. It differs fundamentally from human collagen in genetic sequence, making it a non-homologous substance. This poses risks of rejection, allergies, and viral transmission.

Recombinant Collagen Analogs: Collagen analogs produced through genetic recombination and biotechnology have structural and characteristic similarities to human collagen. However, modifications to their structure may hinder the preservation of all the properties and carry some risk of allergenicity.

Recombinant Human-Type Collagen: With a genetic sequence identical to human collagen, it exhibits excellent tissue compatibility. The immune system recognizes it as a self-substance, facilitating its easy absorption through the skin barrier. It is directly absorbed by the body and actively participates in building collagen in the dermis, ensuring high utility and activity.

Recombinant Human Type III Collagen:

Structure:

Recombinant human Type III collagen has a flexible, curved triple-helical structure with functional regions.

Sequence:

The repeating units of this collagen analog have an amino acid sequence identical to that of human collagen, ensuring high purity.

Integrin Binding:

Recombinant human Type III collagen has abundant integrin recognition sites, such as GPAGEK, GAPGER, and GPAGFR. These sites enable interaction with receptors on the surface of fibroblasts, enhancing integrin binding and further regulating cell recognition, adhesion, and migration. Moreover, the flexible curvature of recombinant human Type III collagen promotes better binding with cell receptor proteins.

High biological activity

*Chen Hua a, Yun Zhu b, Wei Xu, et al. Characterization by high-resolution crystal structure analysis of a triple-helix region of human collagen type III with potent cell adhesion

Test Item	Research Results
Skin Patch	Non-irritating
CAMVA Eye Irritation	Non-irritating
Acute Skin Irritation Test	Non-irritating, no phototoxicity, no skin sensitization observed
Irritation Test Inspection	Complies with the "Medical Device Biological Evaluation" standard
Genetic Mutation Test	Negative for genetic mutations
Acute Systemic Toxicity Test	No observed toxicity, no significant weight differences
Skin Sensitization Test	No skin sensitization reaction observed with test extract
Cell Chromosome Aberration Test	Results of the cell chromosome aberration test were negative
In Vitro Cytotoxicity Test	Test extract showed no cytotoxicity
Subacute Systemic Toxicity Test	No subacute systemic toxicity observed

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